Short-term ubiquinol-10 supplementation alleviates tissue damage in muscle and fatigue caused by strenuous exercise in male distance runners
Abstract
Abstract.Background: Coenzyme Q10 (CoQ10) is the electron transporter in oxidative phosphorylation and an endogenous antioxidant. Recent researches have indicated that doses of 200–300 mg/day are needed to recognize effects to prevent oxidative damage in athletes, and the reduced form of CoQ10, ubiquinol-10, is more bioavailable than its oxidized form. Therefore, we hypothesized that higher doses of ubiquinol-10 could elevate plasma CoQ10 levels rapidly and exert physiological benefits in athletes. Therefore, a placebo controlled, double blinded test was carried out to determine the effects of ubiquinol-10 on the extravasate enzymes and fatigue levels of distance runners. Methods: Sixteen male collegiate distance runners were allocated to two groups receiving 300 mg/day of ubiquinol-10 (19.8 ± 1.7 years) or a placebo (20.1 ± 1.6 years) for 12 days during summer training that comprised 25- and 40-km runs on days 7 and 9, respectively. Results: Ubiquinol-10 elevated plasma CoQ10 concentration to 5.62 μg/mL and significantly decreased activities of the serum extravasate enzymes, CK, ALT, LDH (P < 0.01), and AST (P < 0.05) on day 6. Subjective fatigue status was significantly elevated on day 10 (the day after the 45-km run) in the placebo group (P < 0.001), but did not significantly change in the group given ubiquinol-10. Therefore, ubiquinol-10 could mitigate tissue damage and alleviate fatigue status in distance runners during summer training. Conclusions: Ubiquinol-10 (300 mg/day) supplementation elevated plasma CoQ10 concentrations almost to plateau levels, decreased extravasate enzymes within six days, and suppressed the subjective fatigue in male distance runners.
References
1 . Biochemical Functions of Coenzyme Q10. J Am Coll Nutr. 2001;20:591–8.
2 . Coenzyme Q – Biosynthesis and functions. Biochem Biophys Res Commun. 2010;396:74–9.
3 . Role of coenzyme Q10 (CoQ10) in cardiac disease, hypertension and Meniere-like syndrome. Pharmacol Ther. 2009;124:259–68.
4 . Free radicals and tissue damage produced by exercise. Biochem Biophys Res Commun. 1982;107:1198–205.
5 . Exercise-induced oxidative stress: Cellular mechanisms and impact on muscle force production. Physiol Rev. 2008;88:1243–76.
6 . The effect of muscle-damaging exercise on blood and skeletal muscle oxidative stress: Magnitude and time-course considerations. Sport Med. 2008;38:579–606.
7 . Antioxidant Supplementation during Exercise Training. Sport Med. 2011;41:1043–69.
8 . Exercise, muscle damage and fatigue. Sports Med. 1992;13:108–15.
9 Impact of different running distances on muscle and lymphocyte DNA damage in amateur marathon runners. J Phys Ther Sci. 2016;28:450–5.
10 . Comparison of Changes in Biochemical Markers for Skeletal Muscles, Hepatic Metabolism, and Renal Function after Three Types of Long-distance Running. Medicine (Baltimore). 2016;95:1–6.
11 Clinical impact of speed variability to identify ultramarathon runners at risk for acute kidney injury. PLoS One. 2015;10:1–11.
12 . Effects of 24 h ultra-marathon on biochemical and hematological parameters. World J Gastroenterol. 2004;10:2711–4.
13 Effects of coenzyme Q10administration on its tissue concentrations, mitochondrial oxidant generation, and oxidative stress in the rat. Free Radic Biol Med. 2002;33:627–38.
14 . Coenzyme Q intake elevates the mitochondrial and tissue levels of Coenzyme Q and alpha-tocopherol in young mice. J Nutr. 2003;133:3175–80.
15 Ubiquinol-10 ameliorates mitochondrial encephalopathy associated with CoQ deficiency. Biochim Biophys Acta - Mol Basis Dis. 2014;1842:893–901.
16 . Coenzyme Q10 administration increases brain mitochondrial concentrations and exerts neuroprotective effects. Proc Natl Acad Sci U S A. 1998;95:8892–7.
17 Coenzyme Q10therapy before cardiac surgery improves mitochondrial function and in vitro contractility of myocardial tissue. J Thorac Cardiovasc Surg. 2005;129:25–32.
18 . Assessment of coenzyme Q10 absorption using an in vitro digestion-Caco-2 cell model. Int J Pharm. 2007;333:112–7.
19 . The uptake and distribution of coenzyme Q10. Mitochondrion. 2007;7(Suppl):S72–7.
20 . Comparison study of plasma coenzyme Q10 levels in healthy subjects supplemented with ubiquinol versus ubiquinone. Clin Pharmacol Drug Dev. 2014;3:13–7.
21 . Well-Known Antioxidants and Newcomers in Sport Nutrition: Coenzyme Q10, Quercetin, Resveratrol, Pterostilbene, Pycnogenol and Astaxanthin. 2015.
22 . Coenzyme Q10 supplementation and exercise in healthy humans: a systematic review. Curr Drug Metab. 2016;17:345–58.
23 . Human serum ubiquinol-10 levels and relationship to serum lipids. Int J Vitam Nutr Res. 1989;59:288–92.
24 . Study on safety and bioavailability of ubiquinol (Kaneka QHTM) after single and 4-week multiple oral administration to healthy volunteers. Regul Toxicol Pharmacol. 2007;47:19–28.
25 . Safety assessment of coenzyme Q10 (Kaneka Q10) in healthy subjects: A double-blind, randomized, placebo-controlled trial. Regul Toxicol Pharmacol. 2006;44:212–8.
26 . Increased bioavailability of ubiquinol compared to that of ubiquinone is due to more efficient micellarization during digestion and greater GSH-dependent uptake and basolateral secretion by Caco-2 cells. J Agric Food Chem. 2014;62:7174–82.
27 Reducing exercise-induced muscular injury in kendo athletes with supplementation of coenzyme Q10. Br J Nutr. 2008;100:903–9.
28 . Determination of the ubiquinol-10 and ubiquinone-10 (coenzyme Q10) in human serum by liquid chromatography tandem mass spectrometry to evaluate the oxidative stress. J Chromatogr A. 2007;1175:242–8.
29 . Sensitivity and accuracy of the visual analogue scale: a psycho-physical classroom experiment. Br J Clin Pharmacol. 1978;6:15–24.
30 . Visual analogue scale correlates of musculoskeletal fatigue. Percept Mot Skills. 2004;99:235–46.
31 . Coenzyme Q10 does not prevent exercise-induced muscle damage and oxidative stress in sedentary men. J Sports Med Phys Fitness. 2018;58:889–94.
32 . Studies on Lymphatic Absorption of 1’, 2’-(3H)-Coenzyme Q10 in Rats. Chem Pharm Bull (Tokyo). 1972;20:2585–92.
33 . Absorption, lipoportein transport, and regulation of plasma concentrations of vitamin E in humana. J Lipid Res. 1993;34:343.
34 Free radicals in exhaustive physical exercise: Mechanism of production, and protection by antioxidants. IUBMB Life. 2000;50:271–7.
35 . Effect of Short-ter Coenzyme Q10 Supplementation and Precooling on Serum Endogenous Antioxidant Enzymes of Elite Swimmers. J Strength Cond Res. 2018;32:1431–9.
36 Effect of combined coenzyme Q10 and d-alpha-tocopheryl acetate supplementation on exercise-induced lipid peroxidation and muscular damage: a placebo-controlled double-blind study in marathon runners. Free Radic Res. 1998;29:85–92.
37 Effect of reduced coenzyme Q10 (ubiquinol) supplementation on blood pressure and muscle damage during kendo training camp: a double-blind, randomized controlled study. J Sports Med Phys Fitness. 2015;55:797–804.
38 . Oxygen consumption and usage during physical exercise: The balance between oxidative stress and ros-dependent adaptive signaling. Antioxid Redox Signal. 2013;18:1208–46.
39 . Exercise training-induced Regulation of Mitochondrial Quality. Exerc Sport Sci Rev. 2012;40:159–64.
40 . Exercise and antioxidant supplements in the elderly. J Sport Heal Sci. 2013;2:94–100.
41 . Dietary antioxidants as modifiers of physiologic adaptations to exercise. Med Sci Sports Exerc. 2015;47:1857–68.
42 Ubiquinol-10 Supplementation Activates Mitochondria Functions to Decelerate Senescence in Senescence-Accelerated Mice. Antioxid Redox Signal. 2014;20:2606–20.
43 Ubiquinol effects on antiphospholipid syndrome prothrombotic profile: A randomized, placebo-controlled trial. Arterioscler Thromb Vasc Biol. 2017;37:1923–32.
44 Coenzyme Q10 Prevents Senescence and Dysfunction Caused by Oxidative Stress in Vascular Endothelial Cells. Oxid Med Cell Longev. 2018;2018:3181759.
45 Changes in renal markers and acute kidney injury after Correspondence : ABSTRACT : The impact of marathon running on kidney function has not. Nephrology. 2011;16:194–9.
46 MicroRNAs as biomarkers of hepatotoxicity in a randomized placebo-controlled study of simvastatin and ubiquinol supplementation. Exp Biol Med. 2016;241:317–30.
47 . Beneficial effect of ubiquinol, the reduced form of coenzyme Q10, on cyclosporine nephrotoxicity. Int Braz J Urol. 2012;38:230–4.; discussion 234.
48 Ubiquinol-10 supplementation improves autonomic nervous function and cognitive function in chronic fatigue syndrome. BioFactors. 2016;42:431–40.
49 . Coenzyme Q10 as a treatment for fatigue and depression in multiple sclerosis patients: A double blind randomized clinical trial. Nutr Neurosci. 2016;19:138–43.
50 Increased oxidative stress and coenzyme Q10 deficiency in juvenile fibromyalgia: amelioration of hypercholesterolemia and fatigue by ubiquinol-10 supplementation. Redox Rep. 2013;18:12–9.
51 Antifatigue effects of coenzyme Q10 during physical fatigue. Nutrition. 2008;24:293–9.
52 . An Evidence-Based Approach for Choosing Post-exercise Recovery Techniques to Reduce Markers of Muscle Damage, Soreness, Fatigue, and Inflammation: A Systematic Review With Meta-Analysis. Front Physiol. 2018;9:403.