Lycopene hampers lung injury due to skeletal muscle ischemia-reperfusion in rat model
Abstract
Abstract. This study investigates lycopene’s preventive efficacy in skeletal muscle ischemia-reperfusion (I/R) induced lung injury. Thirty-two rats were randomly assigned to control group, lycopene group, I/R group and I/R + lycopene group. In the lycopene and I/R + lycopene groups, the rats initially received 10 mg/kg/day lycopene orally for 15 days. Then, dissection around the abdominal aorta was performed in all rats under general anesthesia. The aorta was clamped at the infrarenal level in the I/R group and I/R + lycopene group for two hours before two hours of reperfusion. The mean serum levels of malondialdehyde (53.0 ± 20.14 nmol/mL) and superoxide dismutase (1.03 ± 0.16 U/mL) were higher and lower in the I/R group than the other three groups, respectively (p < 0.001). The mean serum IMA level of I/R + lycopene group (0.42 ± 0.04 abs/u) was lower than the I/R group (0.47 ± 0.04 abs/u) (p = 0.015). The mean tissue malondialdehyde levels of I/R group (69.10 ± 11.55 nmol/mL) and I/R + lycopene group (68.36 ± 21.17 nmol/mL) were high compared to the control group (49.87 ± 6.52 nmol/mL) and lycopene group (47.82 ± 4.44 nmol/mL) (p = 0.002). The mean tissue glutathione peroxidase (p < 0.001) and superoxide dismutase (p = 0.001) levels of I/R group (121.81 ± 43.59 nmol/mL and 25.17 ± 8.69 U/mL) were low compared to the control group (236.12 ± 18.01 nmol/mL and 46.30 ± 5.17 U/mL), lycopene group (227.52 ± 16.92 nmol/mL and 45.82 ± 4.02 U/mL), and I/R + lycopene group (176.02 ± 24.27 nmol/mL and 35.20 ± 4.85 U/mL). The histopathological analyses of I/R + lycopene group indicated less significant changes than the control group. Tissue damage in the I/R + lycopene group was less prominent than the I/R group. These findings suggest oral lycopene supplementation as a promising prevention against skeletal muscle I/R caused lung injury.
References
1 Effects of adrenomedullin and vascular endothelial growth factor on ischemia/reperfusion injury in skeletal muscle in rats. J Surg Res. 2013;185(1):56–63.
2 Curcumin protects against ischemia/ reperfusion injury in rat skeletal muscle. J Surg Res. 2012;172(1):e39–46.
3 . Vascular ischemia and reperfusion injury. Br Med Bull. 2004;70:71–86.
4 The effects of iloprost on lung injury induced by skeletal muscle ischemia-reperfusion. Bratisl Lek Listy. 2014;115(7):405–10.
5 . Carotenoids: Biochemistry, pharmacology and treatment. Br J Pharmacol. 2017;174(11):1290–324.
6 Combined effects of curcumin and lycopene or bixin in yoghurt on inhibition of LDL oxidation and increases in HDL and paraoxonase levels in streptozotocin-diabetic rats. Int J Mol Sci. 2017;18(4):332.
7 . Tomato lycopene attenuates myocardial infarction induced by isoproterenol: Electrocardiographic, biochemical and anti-apoptotic study. Asian Pac J Trop Biomed. 2012;2(5):345–51.
8 . Can lycopene be considered an effective protection against cardiovascular disease? Food Chem. 2018;245:1148–53.
9 . Anti-Inflammatory effect of lycopene on experimental spinal cord ischemia injury via cyclooxygenase-2 suppression. Neuroimmunomodulation. 2019;26(2):84–92.
10 . Microsomal lipid peroxidation. Methods Enzymol. 1978;52:302–10.
11 . Assaying for superoxide dismutase activity: Some large consequences of minor changes in conditions. Anal Biochem. 1987;161(2):559–66.
12 . Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967;70(1):158–69.
13 . A novel assay for cobalt-albumin binding and its potential as a marker for myocardial ischemia – a preliminary report. J Emerg Med. 2000;19(4):311–5.
14 . Reactive oxygen species-induced molecular damage and its application in pathology. Pathol Int. 1999;49(2):91–102.
15 . Reactive oxygen metabolites and reperfusion injury: Aberrant triggering of reticuloendothelial function. Lancet. 1994;344(8927):934–6.
16 . Measurement of free radical production by in vivo microdialysis during ischemia-reperfusion injury to skeletal muscle. Free Radic Biol Med. 2001;30(9):979–85.
17 . The inflammatory response to vascular surgery associated ischemia and reperfusion in man: Effect on postoperative pulmonary function. Eur J Vasc Endovasc Surg. 1997;14(5):351–9.
18 . The pathophysiology of skeletal muscle ischemia and the reperfusion syndrome: A review. Cardiovasc Surg. 2002;10(6):620–30.
19 . The effect of ischemia reperfusion injury on skeletal muscle. Injury. 2012;43(6):670–5.
20 Antioxidative effects of adrenomedullin and vascular endothelial growth factor on lung injury induced by skeletal muscle ischemia-reperfusion. Bratisl Lek Listy. 2013;114(11):625–8.
21 . Combined 1,25-dihydroxy-vitamin D and resveratrol: A novel therapeutic approach to ameliorate ischemia reperfusion-induced myocardial injury. Int J Vitam Nutr Res. 2015;85(3–4):174–84.
22 . Inhibition of C5a-induced neutrophil chemotaxis and macrophage cytokine production in vitro by a new C5a receptor antagonist. Biochem Pharmacol. 2000;60(5):729–33.
23 . Oral vitamin C reduces the injury to skeletal muscle caused by compartment syndrome. J Bone Joint Surg Br. 2004;86(6):906–11.
24 Moderation of skeletal muscle reperfusion injury by a sLe(x)-glycosylated complement inhibitory protein. Am J Physiol Cell Physiol. 2001;281(1):C224–30.
25 The effects of erdosteine on lung injury induced by the ischemia–reperfusion of the hind–limbs in rats. J Surg Res. 2008;145(2):303–7.
26 Ascorbic acid (vitamin C) and iloprost attenuate the lung injury caused by ischemia/reperfusion of the lower extremities of rats. Ann Vasc Surg. 2006;20(1):49–55.
27 . Lycopene: hepatoprotective and antioxidant effects toward bisphenol a-induced toxicity in female Wistar rats. Oxid Med Cell Longev. 2018;4:1–8.
28 The short-term protective effects of lycopene on renal ischemia-reperfusion injury in rats. Turk J Urol. 2014;40(1):46–51.
29 The protective effect of adrenomedullin on renal injury, in a model of abdominal aorta cross-clamping. Thorac Cardiovasc Surg. 2012;60(1):5–10.
30 . Cardioprotective activity of silymarin in ischemia-reperfusion-induced myocardial infarction in albino rats. Exp Clin Cardiol. 2007;12(4):179–87.
31 . Quercetin protects rat skeletal muscle from ischemia reperfusion injury. J Enzyme Inhib Med Chem. 2016;31(Suppl 2):162–6.
32 . Tramadol alleviates myocardial injury induced by acute hindlimb ischemia reperfusion in rats. Arq Bras Cardiol. 2015;105(2):151–9.
33 Cardioprotective effect of lycopene in the experimental model of myocardial ischemia-reperfusion injury. Mol Cell Biochem. 2006;289(1–2):1–9.
34 Ischemia-modified albumin levels in cerebrovascular accidents. Am J Emerg Med. 2008;26(8):874–8.
35 . Does the interdependence between oxidative stress and inflammation explain the antioxidant paradox? Oxid Med Cell Longev. 2016;2016:5698931.
36 . The protective effects of lycopene on ischemia/reperfusion injury in rat hind limb muscle model [
Sıçan arka uzuv kas modelinde iskemi/reperfüzyon hasarına yönelik likopenin koruyucu etkisi ]. Ulus Travma Acil Cerrahi Derg. 2020;26(3):351–60.
