Hydro-ethanolic extract of Curcuma longa affects tracheal responsiveness and lung pathology in ovalbumin-sensitized rats
Abstract
Abstract. Anti-inflammatory effect of Curcuma longa (C. longa) was shown previously. In the present study, the effect of the plant on tracheal responsiveness and lung pathological features in ovalbumin-sensitized rats was evaluated. Six groups of rats including control (C), ovalbumin (OVA)-sensitized (S), S groups treated with C. longa (CL; 0.75, 1.50, and 3.00 mg/ml equal to 150, 300 and 600 mg/kg/day) and dexamethasone (D; 1.25 μg/ml) were studied (n=8 in each group). The extract of C. longa and dexamethasone were administered with daily drinking water of animals during sensitization period (for 21 days). Following the treatment period, tracheal responsiveness to methacholine and ovalbumin and lung pathological features was investigated. Tracheal responsiveness to methacholine and OVA and lung pathological scores were increased in group S compared to controls (p<0.01 to p<0.001); however, these parameters in groups treated with dexamethasone and two higher concentrations of C. longa were significantly decreased compared to group S (p<0.05 to p<0.001). Tracheal responsiveness to methacholine was decreased from 50 to 400% due to the extract treatment. All concentrations of C. longa significantly decreased interstitial fibrosis compared to group S (p<0.05 to p<0.001). Treatment with the extract resulted to improvement of pathological changes from 20 to 70%. These results showed a preventive effect for C. longa extract on tracheal responsiveness and lung pathological insults in sensitized rats which were similar or even more than those of dexamethasone at used concentrations.
References
1 (2011) Chemical Composition and Product Quality Control of Turmeric (Curcuma longa L.). Pharmaceutical Crops. 2, 28–54.
2 (2015) Bronchodilatory activity of Curcuma longa, Zingiber officinale and Alpinia galanga based herbal formulation (AHF). Orient Pharm Exp Med. 15, 341–346.
3 (2015) Search for a Herbal Medicine: Anti-asthmatic Activity of Methanolic Extract of Curcuma longa. Res Rev J Pharmacogn Phytochem. 3, 59–72.
4 (2011) Quality and antioxidant properties of bread containing turmeric (Curcuma longa L.) cultivated in South Korea. Food Chem. 124, 1577–1582.
5 (2011) Anti-inflammatory effect of Curcuma longa (turmeric) on collagen-induced arthritis: an anatomico-radiological study. Clin Ter. 162, 201–207.
6 (2011) Evaluation of anxiolytic and muscle relaxant effect of polyherbal formulation of Curcuma longa and Butea frondosa on rats. Pharmacologyonline. 3, 1007–1014.
7 (2008) Involvement of Na+-Ca 2+ exchanger in the endothelium-independent vasorelaxation induced by Curcuma longa L. in isolated rat superior mesenteric arteries. J Smooth Muscle Res. 44, 151–158.
8 (1984) Some aspects of pharmacological profile of sodium curcuminate. Indian J Physiol Pharmacol. 28, 211–215.
9 (1987) Enhanced alveolar cell luminol-dependent chemiluminescence in asthma. J Allergy Clin Immunol. 80, 195–201.
10 (2006) Asthma: pathology and pathophysiology. Arch Pathol Lab Med. 130, 447–451.
11 (2012) Eosinophilic activity and bronchial hyperresponsiveness within an asthmatic pediatric population. Allergol Immunopathol. 40, 301–305.
12 (2017) The effect of hydro-ethanolic extract of Curcuma longa rhizome and curcumin on total and differential WBC and serum oxidant, antioxidant biomarkers in rat model of asthma. Iran J Basic Med Sci. 20, 155–165.
13 (2013) Investigation of antimicrobial and anti-inflammatory activity of Curcuma longa. Int J Pharm Sci Res. 4, 1105–1109.
14 (2013) Hepatoprotective effect of ethanolic extract of Curcuma longa on thioacetamide induced liver cirrhosis in rats. BMC Complement Altern Med. 13, 1–17.
15 (2009) Evaluation of the aqueous extract of Rotula aquatica roots in acute and chronic inflammatory conditions in rats. Pharm Biol. 47, 491–495.
16 (2014) Potential effect of the extract of Zataria multiflora and its constituent, carvacrol, on lung pathology, total and differential WBC, IgE and eosinophil peroxidase levels in sensitized guinea pigs. J Func Foods. 11, 49–61.
17 (2013) Effect of carvacrol on tracheal responsiveness, inflammatory mediators, total and differential WBC count in blood of sensitized guinea pigs. Exp Biol Med. 238, 200–208.
18 (2014) Recent developments in delivery, bioavailability, absorption and metabolism of curcumin: the golden pigment from golden spice. Cancer Res Treat. 46, 2–18.
19 (2007) The effect of exposure of guinea pig to cigarette smoke and their sensitization in tracheal responsiveness to histamine and histamine receptor (H1) blockade by chlorpheniramine. Pathophysiol. 14, 97–104.
20 (1997) Effects of neurokinin depletion on airway inflammation induced by chronic antigen exposure. Am J Respir Crit Care Med. 155, 1739–1747.
21 (2017) Urtica dioica attenuates ovalbumin-induced inflammation and lipid peroxidation of lung tissues in rat asthma model. Pharm Biol. 55, 1561–1568.
22 (2012) Curcuma longa extract exerts a myorelaxant effect on the ileum and colon in a mouse experimental colitis model, independent of the anti-inflammatory effect. PloS one. 7, 1–12.
23 (2003) Curcumin attenuates allergen-induced airway hyperresponsiveness in sensitized guinea pigs. Biol Pharm Bull. 26, 1021–1024.
24 (2015) Curcumin ingestion inhibits mastocytosis and suppresses intestinal anaphylaxis in a murine model of food allergy. PloS one. 10, 1–20.
25 (2013) Intranasal curcumin and its evaluation in murine model of asthma. Int Immunopharmacol. 17, 733–743.
26 (2006) Relaxant effect of Thymus vulgaris on guinea‐pig tracheal chains and its possible mechanism(s). Phytother Res. 20, 28–33.
27 (2010) Curcumin alone and in combination with augmentin protects against pulmonary inflammation and acute lung injury generated during Klebsiella pneumoniae B5055-induced lung infection in BALB/c mice. J Med Microbiol. 59, 429–437.
28 (2009) Preventive effects of curcumin on different aspiration material-induced lung injury in rats. Pediatr Surg Int. 25, 83–92.
29 (2013) Curcumin modulates the inflammatory response and inhibits subsequent fibrosis in a mouse model of viral-induced acute respiratory distress syndrome. PloS one. 8, 1–13.
30 (2013) Curcumin inhibits the proliferation of airway smooth muscle cells in vitro and in vivo. Int J Mol Med. 32, 629–636.
31 (2017) Anti‐inflammatory, antioxidant, and immunomodulatory effects of curcumin in ovalbumin‐sensitized rat. BioFactors. 43, 567–576.